Optimal Culture Conditions for Mycelial Growth and Fruiting Body Formation of Ling Zhi Mushroom Ganoderma lucidum strain GA3

Bich Thuy Thi Nguyen1, Nghien Xuan Ngo1, Ve Van Le1, Luyen Thi Nguyen1, Ry Kana1, Huy Duc Nguyen2

1Faculty of Biotechnology, Vietnam National University of Agriculture

2Faculty of Agronomy, Vietnam National University of Agriculture

Received 17 November 2018; accepted 12 February 2019



The objective in this study is to investigate optimal culture conditions for mycelial growth and fruiting body formation of the Ling Zhi mushroom, Ganoderma lucidum strain GA3. The results of the study show that the optimal media and temperature for the mycelial growth are potato, glucose, and agar (PGA) supplemented with rice bran, and 25-30oC, respectively. Strain GA3 is able to grow in a wide pH range, between 4 and 12. The most favourable substrate mixture for the formation and development of the fruiting body is 87% sawdust + 4% corn powder + 8% rice bran + 1% calcium carbonate (CaCO3).

Keywords: fruiting bodies, Ling Zhi mushroom, media, mycelium.