Partitioning of Fluoxetine into Mixed Lipid Bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)
Anh T. Sy, Vy T. Pham, Trang T. Nguyen*
School of Biotechnology, International University, Vietnam National University in Ho Chi Minh city
Received 10 August 2018; accepted 3 April 2019
In this study, the partitioning of fluoxetine, an antidepressant of selective serotonin reuptake inhibitor class into a mixture containing anionic and zwitterionic lipid vesicles was evaluated using second derivative spectrophotometry. The partition coefficients (Kp) of fluoxetine into the large unilamellar vesicles (LUVs) composed of zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) containing 0 mol%, 10 mol%, 20 mol%, and 30 mol% of anionic 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) were measured in HEPES buffer at pH 7.4. The result revealed that when more negatively charged lipids incorporated into the LUVs, the condensing effect on the binary phospholipid membrane impeded the partitioning of positively charged fluoxetine, resulting in the decrease in the Kp values. This study adds a deeper understanding of how antidepressant fluoxetine exerts its effect on anionic-containing biological membranes, shedding light onto drug delivery systems in the pharmaceutical field.
Keywords: binary phospholipid membrane, electrostatic interaction, fluoxetine, partition coefficient, second derivative spectrophotometry.