Main Article Content
Liposomal amphotericin B were prepared by thin film hydration technique. Particle size was reduced by ultrasonic device and high-pressure homogeneous device. The size distribution was determined by dynamic light scattering by the Zetasizer ZS90 equipment. The morphology of amphotericin B liposomes was observed by Transmission electronic microscopy (TEM) with negative staining technique. The result shows that combining high-pressure homogenization and membrane extrusion provided monolayer liposomal amphotericin B with the particle size less than 200 nm and homogeneously (PDI < 0.3).