Establishment of multiplex PCR for detection of genes related to Quinolone resistance

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Ngo Tat Trung Dao Thanh Quyen Phan Quoc Hoan Thirumalaisamy P.P. Velavan Le Huu Song

Abstract

Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets. This study aimed to optimise a multiplex PCR assay to track plasmid-mediated low-level quinolone resistance profiles.
Subjects and methods: A multiplex PCR-based-method in which the primers were already established by our team. About 44 samples were collected from 44 patients who enrolled in this study by using surgical site infection (SSI).
Results: By targeting the conserved domains of qnrA, qnrB, qnrS and the qnrVC gene families, the primer number was reduced significantly to only four pairs in one multiplex PCR. Using multiplex PCR, 3/44 SSI samples were found to be carrying fluoroquinoloneresistance genes (qnrA, qnrB, qnrS, qnrVC).
Conclusion: A multiplex PCR for detecting pathogens as well as identifying quinolone resistance genes all in one reaction was successfully established.

 

DOI: https://doi.org/10.31276/VJSTE.60(2).51

Article Details

How to Cite
TRUNG, Ngo Tat et al. Establishment of multiplex PCR for detection of genes related to Quinolone resistance. Vietnam Journal of Science, Technology and Engineering, [S.l.], v. 60, n. 2, p. 51-55, june 2018. ISSN 2525-2461. Available at: <http://vietnamscience.vn/index.php/VJSTE/article/view/121>. Date accessed: 19 july 2018.
Section
LIFE SCIENCES