DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam

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Manh Minh Bui Anh Tuan Vu Phuong Nhung Vu Quang Cu Pham Dang Ton Nguyen Thi Thu Hue Huynh

Abstract

Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives. DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L. samples collected from the mountain areas of Northern Vietnam. Based on the combined sequence data of ribosomal nuclear ITS, a part of chloroplast rbcL gene and trnL-trnF intergenic spacer, phylograms of Scrophularia L. were generated by both Bayesian inference and maximum likelihood bootstrap method. The phylogenetic analysis showed that the tested samples have a sister relationship to S. ningpoensis. Hopefully, the analysis strategy that we used would contribute to further phylogenetic analyses of medicinal plants of Vietnam in the future.

 

DOI: https://doi.org/10.31276/VJSTE.60(2).56

Article Details

How to Cite
BUI, Manh Minh et al. DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam. Vietnam Journal of Science, Technology and Engineering, [S.l.], v. 60, n. 2, p. 56-64, june 2018. ISSN 2525-2461. Available at: <http://vietnamscience.vn/index.php/VJSTE/article/view/122>. Date accessed: 19 july 2018.
Section
LIFE SCIENCES