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The objective in this study is to investigate optimal culture conditions for mycelial growth and fruiting body formation of the Ling Zhi mushroom, Ganoderma lucidum strain GA3. The results of the study show that the optimal media and temperature for the mycelial growth are potato, glucose, and agar (PGA) supplemented with rice bran, and 25-30oC, respectively. Strain GA3 is able to grow in a wide pH range, between 4 and 12. The most favourable substrate mixture for the formation and development of the fruiting body is 87% sawdust + 4% corn powder + 8% rice bran + 1% calcium carbonate (CaCO3).