Main Article Content
Cinnamon (Cinnamomum cassia B.) is an important crop in Vietnam for domestic consumption and exportation. In recent years, a disease known as cinnamon witches’ broom (CinWB) has been discovered on cinnamon grown in Tra Bong district - Quang Ngai province. The typical symptoms of CinWB were the formation of small tumors on the stems, branches, petioles, and veins of plants. The tumors become long squid-like tassels giving the appearance of a witches’ broom. Infected plants are stunted, with delayed growth and development, causing a high reduction in the yield and quality of farmed cinnamon. In the present study, nested-PCR was applied with the universal primer pairs P1/P7 and R16F2n/R16R2. PCR products of an approximate size of 1200 bp were amplified from the twelves CinWB-showing samples collected from Tra Bong district, Quang Ngai province. All PCR products were directly sequenced in both directions using R16F2n and R16R2 primers. A BLAST search indicated that DNA sequences of all 12 PCR products were identical and show 99% identity with phytoplasma sequences of the 16SrXIV group. And the CinWB phytoplasma isolated from the CinWB-showing cinnamon from Tra Bong district - Quang Ngai province (QQNVN) was deposited in GenBank under an accession number JX413793.
How to Cite
DUONG, Thi Nguyen; DAO, Thanh Van. Molecular detection and identification of a phytoplasma associated with cinnamon (Cinnamomum cassia B.) witches’ broom disease in Quang Ngai province, Vietnam. Vietnam Journal of Science, Technology and Engineering, [S.l.], v. 59, n. 3, p. 44-47, sep. 2017. ISSN 2525-2461. Available at: <http://vietnamscience.vn/index.php/vistech/article/view/68>. Date accessed: 15 dec. 2017.